Sensitive and selective chem/bio sensing based on surface-enhanced Raman spectroscopy (SERS)
نویسندگان
چکیده
This paper summarizes our recent progress toward developing anthrax and glucose sensors based on surface-enhanced Raman spectroscopy (SERS). Ag film over nanosphere (AgFON) substrates was used as the SERS sensor platform in both cases. The AgFON substrates have been optimized for near-infrared (NIR) laser excitations by tuning the extinction maximum of their localized surface plasmon resonance (LSPR). Bacillus subtilis spores, harmless simulants for Bacillus anthracis, were studied using SERS. Calcium dipicolinate, a biomarker for bacillus spores, was efficiently extracted from spores and rapidly detected by SERS. A limit of detection (LOD) of 2.6 10 spores, below the anthrax infectious dose of 10 spores, has been achieved within 11 min. For glucose detection, a mixed decanethiol (DT)/mercaptohexanol (MH) partition layer is used to bring glucose closer to the AgFON surface. Quantitative detection of glucose in bovine plasma, as well as complete and rapid partitioning and departitioning, was demonstrated. The root mean square error of prediction (RMSEP) is 83.16 mg/dL (4.62 mM) with 85% of the validation points falling within the A and B range of the Clarke error grid. # 2006 Elsevier B.V. All rights reserved.
منابع مشابه
Biosensing Based on Surface-Enhanced Raman Spectroscopy by Using Metal Nanoparticles
Surface-enhanced Raman spectroscopy (SERS) is a promising tool in the analytical science because it provides good selectivity and sensitivity without the labeling process required by fluorescence detection. This technique consists of locating the target analyte on nanometer range of roughed Au-nanoparticles. The presence of the metal nanoparticles provides a tremendous enhancement to the result...
متن کاملMolecular Diagnosis of Plasma Phenylalanine in Neonates with Phenylketonuria Disease Using Biological Sensors Based on Surface-Enhanced Raman Spectroscopy (SERS)
In this study, silver nanoparticles were chemically synthesized and deposited on glass substrates using a reducing agent of sucrose, at 50°C. Different characterizations including atomic force microscopy (AFM), field emission scanning electron microscopy (FESEM), and Raman spectroscopy were obtained to study silvery substrates. Then, the silvery substrates were used as the SERS substrates to de...
متن کاملUnification of Surface Enhanced Raman Spectroscopy of Dyes Using One Pot Synthesized Stabilized Ag Nanoparticles
stabilized Ag Nanoparticles (NPs) were synthesized using Lee-Meisel method under three different conditions in an oil bath. UV-Vis spectroscopy of the Ag NPs showed a Localized Surface Plasmon (LSP) band around 430 nm, indicating Ag NPs had a size range around 40 nm. To fabricate a surface Enhanced Raman Spectroscopy (SERS) substrate, LSP properties of Ag NPs was employed with the goal of detec...
متن کاملGlass Nanopillar Array Based Nanoplasmonic Lab-on-a-chip for Highly Sensitive Surface Enhanced Raman Spectroscopy
ABSTRACT This work presents the novel glass nanopillar array based nanoplasmonic lab-on-a-chip for highly sensitive surface-enhanced Raman Spectroscopy (SERS). In this work, large-area glass nanopillar arrays are fabricated on both sides of the wafers by combination of metal dewetting and reactive-ion-etching (RIE) process. Metal layer is deposited onto one side to construct nanoplasmonic biose...
متن کاملMagnetic separation and immunoassay of multi-antigen based on surface enhanced Raman spectroscopy.
A novel and highly sensitive immunoassay method based on surface enhanced Raman spectroscopy (SERS) and magnetic particles has been developed. This method exhibits great potential application in bio-separation and immunoassay.
متن کامل